Journal: Kidney360

Article Title: Natural Killer Lymphocytes Mediate Renal Fibrosis Due to Acute Cardiorenal Syndrome

doi: 10.34067/KID.0000000000000305

Figure Lengend Snippet: Kidney mesenchymal cells expand after CA/CPR. (A) Representative flow cytometry plots of PDGFRβ+ mesenchymal cells in the kidney after CA/CPR. Plots shown are gated on live, single cells. (B) Quantification of (A). (C) Representative flow cytometry plots of CD45+PDGFRβ+ mesenchymal cells in the kidney at different time points after CA/CPR. (D) Quantification of (C). (E) Representative 20× immunofluorescence images of PDGFRα/β staining at different time points after CA/CPR. (F) Representative 20× immunofluorescence images of αSMA staining at different time points after CA/CPR. (G) Quantification of (F). (H) qRT-PCR for Acta2 (codes for αSMA) and Col1a1 (codes for type 1 collage) in the kidney at 49 days after CA/CPR compared with sham. *P < 0.05, ***P < 0.001 compared with sham in one-way ANOVA. n=4–8/group. Scale bars=100 μm. CA/CPR, cardiac arrest/cardiopulmonary resuscitation; #αSMA+, number of αSMA positive cells per high-powered field; αSMA, α smooth muscle actin; PDGFRα/β, platelet-derived growth factor α and β.

Article Snippet: Sections were then incubated with primary antibody (rabbit anti-PDGFR α / β , Abcam ab32570, 1:1000, and rat anti-CD45, BD Biosciences 550539, 1:1000) in 1% bovine serum albumin in 0.01 M PBS and 0.1% Triton X-100 at 4°C overnight.

Techniques: Flow Cytometry, Immunofluorescence, Staining, Quantitative RT-PCR, Derivative Assay

Journal: Kidney360

Article Title: Natural Killer Lymphocytes Mediate Renal Fibrosis Due to Acute Cardiorenal Syndrome

doi: 10.34067/KID.0000000000000305

Figure Lengend Snippet: Immune cells colocalize with mesenchymal cells during AKI-CKD transition. Representative 40× immunofluorescence images of different time points after CA/CPR, including 3 days (A), 7 days (B), and 49 days (C). CD45 used to label immune cells, and PDGFRα/β used to label mesenchymal cells. Scale bar=100 μm.

Article Snippet: Sections were then incubated with primary antibody (rabbit anti-PDGFR α / β , Abcam ab32570, 1:1000, and rat anti-CD45, BD Biosciences 550539, 1:1000) in 1% bovine serum albumin in 0.01 M PBS and 0.1% Triton X-100 at 4°C overnight.

Techniques: Immunofluorescence